Δημοσιεύσεις σε Επιστημονικά Περιοδικά

Food Bioscience

Α novel method for the efficient simultaneous quantification of 67 phytoestrogens in plants and foodstuffs

Myrtsi E.D., Koulocheri S.D. & Haroutounian S.A., Food Bioscience 2023, doi: https://doi.org/10.1016/j.fbio.2023.103357.

• A quantitative analytical method, LC-MS/MS, was developed for the simultaneous analysis of 67 phytoestrogens.
• Validation confirms method’s suitability for animal and plant samples.
• The developed method is applied to plant samples belonging to the Fabaceae family.
• Phytoestrogens are quantified in feeds and detected in chicken meat and fish fillet.

ΔΗΜΟΣΙΕΥΣΗ -Food Bioscience

Abstract

Abstract
The significant beneficial effects of phytoestrogens on human and animal health have emerged the necessity to develop a sensitive accurate and rapid method for their efficient quantitation in human and animal foods. Study herein concerns the development of a novel quantitative analytical method using high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) that allows the simultaneous quantitation of 67 natural phytoestrogens. The novel method was fully validated through the determination of the following parameters: selectivity, sensibility, recovery, precision, accuracy of detection and quantification limits for each analyte. Specifically, the novel method proved accurate displaying linear response with R2 values ≥ 0.9991, quantitation limits from 5.5 to 331.8 ng/mL for 98.5% of analytes, recovery values ranging between 64.8% and 150.3%, while the precision and accuracy scores remained below 20% for most analytes. The method was successfully applied on the following plant samples used in human and/or animal diet belonging to Fabaceae plant family: Trifolium repens, Vicia sativa, Pisum sativum and Glycine max (soybean meal). Furthermore, the novel method was applied for the determination of the phytoestrogenic content of two animal feeds (for livestock and fishes) and two foodstuffs (chicken meat and fish fillet).

ΔΗΜΟΣΙΕΥΣΗ – MOLECULES. PDF

 

Abstract

The intensive use of plant materials as a sustainable alternative for fish feed production, combined with their phytochemical content, which affects the growth and production characteristics of farmed fishes, necessitates their monitoring for the presence of raw materials of plant origin. This study reported herein concerns the development, validation and application of a workflow using high-performance liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) for the quantification of 67 natural phytoestrogens in plant-derived raw materials that were used to produce fish feeds. Specifically, we verified the presence of 8 phytoestrogens in rapeseed meal samples, 20 in soybean meal samples, 12 in sunflower meal samples and only 1 in wheat meal samples in quantities enabling their efficient incorporation into clusters. Among the various constituents, the soybean phytoestrogens daidzein, genistein, daidzin, glycitin, apigenin, calycosin and coumestrol, as well as the sunflower neochlorogenic, caffeic and chlorogenic phenolic acids, displayed the highest correlations with their origin descriptions. A hierarchical cluster analysis of the studied samples, based on their phytoestrogen contents, led to the efficient clustering of raw materials. The accuracy and efficiency of this clustering were tested through the incorporation of additional samples of soybean meal, wheat meal and maize meal, which verified the utilization of the phytoestrogen content as a valuable biomarker for the discrimination of raw materials used for fish feed production.